Coding
YLcwp3

Part:BBa_K1592002:Design

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-08-31)


Yarrowia lipolytica cell wall protein 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 35


Design Notes

No


Source

The source of the part will be its sequence which was retrieved from GenBank, and altered some regions by our lab.

References

//It's a short plasmids with known seuence.So we choose PCR to realize the point mutation. we design forward primer as cgaggccgctgctggtgctcacgccactgccggtgccattg and reverser primer as caatggcaccggcagtggcgtgagcaccagcagcggcctcg.Then it's easy to do following steps: running gel, gel extraction, sequencing